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* Faust from ''[[Guilty Gear]]'' before and after his [[Heel Face Turn]], although after he's more like a wild, [[Cloudcuckoolander|mad]], [[The Atoner|but generous]] [[Heroic Sociopath]].
* ''[[The Elder Scrolls]]'' has several, but Relmyna of ''Shivering Isles'' stands out in particular. Obsessed with the power of flesh (no, really), she enjoys creating twisted monstrosities such as Flesh Atronarchs and the Gatekeeper, and considers them her "children". She also conducts some grisly experiments on the concepts of pain and suffering. Oh, and [[Heroic Sociopath|she's on your side]].
* ''[[World of Warcraft]]'' has two ''Wrath of the Lich King'', both undead. Grand Apothecary Putress of the Forsaken performs unholy experiments to create a new [[The Virus|Plague]] to destroy both the undead Scourge and all life in general. Professor Putricide of the Scourge (an obvious parody of Professor Farnsworth from ''[[Futurama]]'') tries to do...basically the same thing, but without the 'destroy the Scourge' part. He also fits the trope better for having a [[Super
** Great news, everyone! Professor Putricide's become something of an [[Ensemble Darkhorse]] since his introduction to the game. [[Memetic Mutation]] probably helps.
** The goblin race as a whole fits this, too. Usually their inventions involve [[Stuff Blowing Up]].
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* Dr. Barbara "Babs" Blight on ''[[Captain Planet and the Planeteers]]'' is literally mad. She is pretty much insane and she is a brilliant scientist, and represents unethical scientific research and technology in the wrong hands, though sometimes, she simply causes environmental damage [[For the Evulz|for the sake of doing so]].
** Some of her plans have a [[Freudian Excuse]] related to her [[Good Scars, Evil Scars|facial scar]], which can actually make her [[Unintentionally Sympathetic]] to some viewers.
* ''[[Star Wars: The Clone Wars]]'': Dr. Nuvo Vindi.
* Doctor Krieger from ''[[Archer]]''.
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* Crossing this trope with [[Magitek]] is ''[[Wakfu]]'''s season one [[Big Bad]] Nox, a watchmaker who discovered that the most effective way of powering [[Clock Punk|his inventions]] was [[Powered by a Forsaken Child|draining]] [[Life Energy|the Wakfu]] [[Powered by a Forsaken Child|out of living organisms]]. A sampling of his creations include [[Vampiric Draining]] [[Literal Surveillance Bug]]s, [[Spider Tank|ambulatory pocket watches]], [[Killer Robot]]s literally powered by the residual substance of ''dead souls'' (generated in the first place by Nox's constant killing for wakfu), {{spoiler|[[Tear Jerker|animatronic replicas of his dead children]]}}...
* In ''[[Beetlejuice (animation)|Beetlejuice]]'', one-shot character [[Punny Name|Dr. Prankenstein]], the most renowned Mad Scientist in the Netherworld.
* The Evil Scientist [[Card-Carrying Villain|(as he called himself)]] from the ''[[Looney Tunes]]'' short "Water Water Every Hare". He wanted to transplant Bugs' brain into a giant robot for some nefarious plan, and was pure [[Nightmare Fuel]].
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