Big No/Web Animation

"Grif: NOOOOO!
 * Grif of Red vs. Blue spends the last moments of the third season screaming "No-!" (seemingly for hours, as said by Donut.), after the Red Team This extends to the the entire first episode of the fourth season, with lots of ridiculous plans (devised by Sarge, who else?) that attempt to force him off the cliff he's perched on. The plans are, in order, allowing him to scream until he passes out.

Sarge: Simmons! Find a way to get Grif back down here right now!

Simmons: Alright. HEY GRIF! SHUT THE FUCK UP AND GET DOWN HERE! WE NEED TO CHECK OUT THE BASE!

Grif: NOOO! NOOOOOO! NOOOOOOOOOOO!

Simmons: I don't think he's listening, sir.

Grif: NOOO! THAT TIME I WAS ANSWERING YOUR QUESTION! NOOOOO!

Sarge: That does it, Simmons, go get him down now.

Donut: Sarge, if he keeps screaming like that, he'll pass out, and fall off the cliff!

Sarge: Cancel that order Simmons!

Grif: NOOOOOOOO!

Sarge: (sighing) Donut, get me a sniper rifle."


 * Strong Mad gets one of these in an encounter with Senor Cardgage on Homestar Runner.
 * Marzipan gets to say one after  in a Halloween special. Since she read the cue card incorrectly, her first attempt sounds more like, "moo," with an "n," than, "no!"