Batman: Mask of the Phantasm/Tear Jerker

"Alfred:"
 * "I didn't count on being happy." - Batman pleads with his (dead) parents and himself to let himself go from the path of vengeance of vigilantism in exchange for happiness with Andrea.
 * The flashback, in which Bruce receives the "Dear John" letter.
 * Alfred's shocked and despairing "My God!" when he first sees Bruce in full costume.
 * Even worse is the moment right before.
 * The last two lines, which sum things up for both Batman and . "Do you wanna be alone?" "I am."
 * Bruce's despair at the end and Alfred's speech also counts: