Astro Boy (film)/Tear Jerker

Original Manga'

 * Hoshie's death.
 * Tenma finally giving up on his schemes to reclaim Astro as his son: "I'm sorry... I give up... I just wanted Astro back, thst's all... I thought if I could get a brand-new Astro, he could be my son again...  I know it was a crazy thing to do... but when I learned I couldn't have a brand-new Astro, I thought maybe I could get you to let me have the old one... I'm sorry... I know it's not possible... you win... I know you're his real mom and dad now... you're both wonderful parents... I know you can make him happy... I'll never come here again... I promise... give my regards to Astro..."

1963 anime

 * Dr. Tenma/Boyton's reaction to his son's death... the worst being when we cut to him in his office, sobbing into his desk...
 * And then later on in the episode is his rejection of Astro. Goes from saying he'll teach his son how to fly and jump mountains to ordering him angrily to get out.
 * From Snow Lion: "This is how it ends: in the snow. If it's the last thing I do, I'll put Astro Boy in the Robot Hall of Fame. Yes, Astro Boy, you've earned your place here with the best and here you'll stay for eternity... and here I'll wait for you, my friend. We'll face the end together."

1980 anime
"Tenma: (calling for him) Tobio, I'm sorry. You are my child.
 * Tenma's desperate search for Astro in the second episode. Curse you, Hamegg!

Astro: (trapped in a suitcase) Father... I'm here... Help me...(slips into unconsciousness)"

2003 series

 * Astro's 'death', short as it may last.
 * The hug between  in the final episode.

2009 film

 * It's the thing you expect the writers to have just glossed over... gets fully shown...
 * Granted, they completely changed Astro's cause of death (he gets hit by a car in both the manga and some of the adaptations), but it otherwise might have been too scary for kids.
 * May have been Meaningful Echo, given the climax of the film.
 * There's also a remote possibility that Toby was absorbed by the Peacekeeper. I was somewhat expecting that as a kid-friendly ending.
 * The aforementioned Please Wake Up scene also brings tears.
 * Tenma's collapse after