Big No/Fan Works

"Jack: COME ON, CROW, LET'S GO PLAY A CARD GAME, WHILE STANDING COMPLETELY STILL...ON. THE. GROUND.
 * In Tales for the Leet's Romeo and Juliet, Juliet has an especially Big No after finding Romeo dead, taking three lines of dialogue to complete and ending with multiple !s and 1s.
 * In Yu-Gi-Oh the Abridged Series, Tristan gets one that the credits cut off after Joey tells him that they have to go back to school now that the tournament is over.
 * Yu-Gi-Oh Tenth Anniversary Movie Abridged About card games on motorcycles:

Yusei: NNNNOOOOOOOOOOOOOOOOOOOOO"

"Goku: Kaioken...
 * My Immortal uses it a lot.
 * In Dragonball Z Abridged:
 * From episode 10:

Vegeta: Noooo!

Goku: ... times...

Vegeta: No no no no!

Goku: ... FOUR!

Vegeta: No no no no FUUUUUUUUUUUUUUUUUUUUUUUUUUUuuuuuuuuuuuuuuuuuuu...!!!!!"


 * Additionally, Vegeta's response to.
 * z3ll58 does a very good and long No in his "Let's Play Final Fantasy 6" when Leo dies.
 * The beginning of Christian Humber Reloaded, when the title hero sees his owners killed. He then follows this up with "WHOEVER DID THIS WILL PAY DEARLY, BECAUSE I LOVED THEM?"
 * Happens at least once per chapter in ''Light and Dark - The Adventures of Dark Yagami, since people are constantly "sexing" and dying and coming back to life and dying again.
 * Starkits Prophecy has one of these about once every other chapter later in the story. Or, due to Rouge Angles of Satin, a big "MOOOOO" or "OOOOOO." Or possibly "NOOOOOO999999999 OOOOOOOO".
 * In A Day in the Life of a Commissar, a Dawn of War Machinima, Commissar Steeve does this when he's told Galactic Starbucks is out of coffee. He stops to ask "Really? What kind?" once he's told that there is, in fact, some coffee left. And finishes when he's told it's decaf.
 * In Deserving, everyone has No Indoor Voice especially when uttering the word "no", which appears in ALLCAPS as often as not.
 * In Revenge Road, Kyosuke wakes up after realizing that the previous events, Hikaru killing him and Madoka was All Just a Dream. Madoka then gets up to answer the door, like she did before, and when Kyosuke realizes what time it is, he runs down the stairs yelling "Midnight? Oh no! Madoka! StOP! NOOOOooooooo!", only to find that he is too late as Madoka gets shot and the fic ends.
 * In Modurn Warfare 3 The Dead Rise, gets an extremely long one as he sees   die, and  gets one when he's decapitated and eaten by a fellow prisoner.
 * This is used quite frequently in Naruto Veangance Revelaitons, but it's seldom rendered as "NOOOOO!". For example, after seeing, Sakura yells "MOOOOOO"!
 * Might Guy yells out "NOOOOOOOOOOO" when he learns that Rock Lee has slipped out of his hospital room.
 * This occurs twice in Oh God Not Again; once by Fred and George when they see the Quiddich field is now a hedge and again by Harry when he finds out Hermione won fifty points (again) after he dedicated himself to losing the House Cup.
 * Nyx's dramatic "No! No no no no no NOOOO!!!" in chapter 3 of Past Sins definitely qualifies, not to mention her many other Rapid Fire Nos.
 * In the Tamers Forever Series
 * Justified in that.
 * D.C also let's out a Big No while.