Transformers Prime/Tear Jerker

"Optimus: We must not allow our anger over the loss of Cliffjumper to impair our judgement. As of today, only we five Autobots remain on this earth. We owe it to ourselves... to the memory of Cybertron... to any Autobots in any galaxy seeking safe harbor... to humankind... and we owe it to the memory of our fallen comrade... to survive."
 * Cliffjumper's death in the first episode. Especially the Autobot's reactions (special mention to Arcee) and Optimus' speech in his memory. It's very clear that he's trying to keep Arcee from running off and doing something stupid, and every iota of sadness and pain is clearly distinguishable in his voice.

"Optimus: Had Skyquake chosen to stray from his master's path... we might not be burying him today."
 * Skyquake's death in "Masters and Students." We see a shot of one of his eyes closing for the last time before a visibly saddened Optimus really drives it home.

"Ratchet: Optimus... I've been a fool..."
 * In "Scrapheap", Arcee and Optimus are stuck dying of robo-hypothermia in the Arctic. As they begin to succumb to the cold, Arcee reaches up and holds Optimus's hand.
 * Arcee's terrifying flashback in "Predatory" of Tailgate's murder at the hands of Airachnid, especially the part when she helplessly cries his name after he dies.
 * Miko and Bulkhead trapped underground in "Rock Bottom", with Miko having a major breakdown.
 * In particular, seeing Miko rendered speechless out of worry, fear and sheer exhaustion is particularly touching, even for some viewers who don't like her.
 * She cried!
 * Arcee talking to her dead partner Cliffjumper at the start of "Partners".
 * Arcee discovering that Starscream was the one who murdered Cliffjumper.
 * From the same episode, Bumblebee's look of sheer disappointment upon seeing Arcee about to kill Starscream.
 * Episode 21: Miko, first when she realizes that Bulkhead's losing his memory of her, and later when it looks like he's been left brain dead.
 * Episode 22- Ratchet confessing his sins while almost bleeding to death. Much crying was had by all.

"Arcee:"
 * Raf being held on life support after being shot by Megatron. He was teetering on the edge of life.
 * Bumblebee's face when he saw him was heartbreaking. Not to mention it's one of the few times in which he almost rushes out to try and kill Megatron for doing it.
 * Add to that how Ratchet berates himself for being too prideful to bother learning about human science and medicine as he cannot find a way to treat Raf, and then pretty much confessing how attached he's gotten to Raf, which could also be seen in the previous episode, when Ratchet invited Raf to watch as he tested synthetic energon.
 * Bumblebee and Raf looking sadly at one another as Raf is driven away in "One Shall Rise, Part 1" is quite heart-wrenching. Thankfully, it's not too long before they're reunited.
 * The end of "One Shall Rise, Part 3". Megatron  and then summons a GroundBridge to the Nemesis. Before  goes through, he sees the faces of, and is completely unable to recognise them. Bumblebee's face is once again the saddest part.
 * Especially the way Bumblebee hopelessly beeps
 * Seeing the Band of Brothers
 * Arcee's reaction to was extremely powerful.

"Ratchet: Yes, well... the medic could have done better."
 * The way This leads to a Moment of Awesome (and a possible Moment Of Funny) when
 * To elaborate on his treatment a little further: he's eventually . Simply seeing reduced to begging fearfully, however briefly, will certainly strike the heart of any Transformers fan.
 * Ratchet telling Raf about how the field medic who saved Bumblebee's life after Megatron tore out his voicebox and left him for dead wasn't able to fix Bumblebee's voice. Of course, he's speaking about his OWN inability to completely fix Bumblebee, since he was the "field medic" who saved Bee's life.


 * Hell, Bumblebee's depression was quite sad too.
 * Followed by Ratchet's heartwrenching plea to donate his T-Cog to Bumblebee, saying he rarely needs it anyways. Both the viewers and Optimus instantly see this as Ratchet's way of trying to atone for not being able to fix Bumblebee before.
 * Bumblebee's utter despair at
 * A subtle one in "Loose Cannons" when Bulkhead has a Hope Spot that another Wrecker is alive. Then Wheeljack says that Dreadwing killed him.
 * "Crossfire" gives us 's death, which manages to be horrific and heartbreaking at the same time. An Insecticon pins his arm with webbing and   The worst part of all, though, is that   Even he deserved better than that.
 * Optimus being gutted by Silas controlling Nemesis Prime. Thank the Matrix he recovered.
 * In "Toxicity", Bulkhead  throughout the episode can be pretty hard to watch. Also.
 * Bulkhead's flashbacks. Dear lord, the flashbacks.