Yukari Is Free/Tear Jerker


 * 's death in Season 3.
 * Rei Kakizaki's entire backstory from the start of the Zombie Apocalypse up to Season 3.5 could easily qualify, but special mention goes to her reaction to Made even worse by the knowledge that she really did believe that  had been the only person left in the world who loved and cared about her, and that now she was completely, utterly alone.
 * And speaking of Rei, in the Discussion Threads, there are occasionally pictures of the entire cast as analyzed by certain cast members. This one, done by Rei, has a rather heartbreaking moment when she gets to Kagura's Mother's picture:
 * While it counts as a Crowning Moment of Awesome, would totally count as this back in Season 2.