Big No/Web Comics

"Elan: Hey, did someone in here just drop to their knees and scream, "Noooooo"? I got a feeling...."
 * Eight Bit Theater:
 * The aptly titled Episode 400: "'Not Safe for Work, School, or Anywhere You Can Get in Trouble" replaces the Big No with the Big F, or rather the Big "FFFFFFFFFFFFFFFFFFFFFFFF UCK! !".
 * Red Mage uses this as Black Mage is about to burn the Rat's Tail they need for the class change. He puts on a levitation spell so he can do the slo-mo jump (but applies too much and is left hanging for a while), and does the Big No just to be dramatic.
 * Darths and Droids has several of these.
 * Dominic Deegan also has a big "FUUUUCK!" moment, too. Made all the funnier because it's rare to see him lose his cool like that.
 * Tedd's father uses this in El Goonish Shive, when he's realizes he's said he'll pay for eight teenagers to eat pizza, while at a meeting far away. The person he's talking to thinks her ideas are being criticized. The comic is helpfully titled 'I Graduated from the George Lucas Directing Academy'.
 * Subverted in the third panel of this strip of Sluggy Freelance, where a "Little No" gives the dramatic impact a Big No is supposed to have.
 * The Order of the Stick
 * Vaarsuvius uses it in strip #504, titled "That's a Surprisingly Common Reaction". Lampshaded in the first panel of the next strip, by Genre Savvy Elan:

"Xykon: No no no no no no NO!
 * Durkon gets one of these in On the Origine of PCs after tasting what is allegedly the "finest beer brewed in all the human lands."
 * And the intermission comic, where... well just see for yourself.
 * V does it again after.
 * Xykon, of all people, does it when his.

[...]

Xykon: YES!"

"Professor Utonium: What's that you got there? Is that a... Wh--Th--P...P....
 * Captain SNES has had its fair share of them:
 * Dr. Wily does the traditional NOOOOOOOOOOOOOO! When he realises he's in a catch 22 as the VP of Nexus and doing anything to ruin Nexus would cause Blues to torture him, meaning he would have to go against his nature and save it from the stupidity that was President Haggar.
 * Alex Willams, replaces the Big No with the Big FUCK, and purposely extends it to buy time to think.
 * He did a simple Big No before. It lasted something like thirteen comics.
 * Shortpacked!:
 * This is Robin's reaction to finding out that Felix Gaeta is portrayed by the same man that voiced Nightscream from Transformers: Beast Machines.
 * This is also Amber's reaction to finding out that  It takes her two comics.
 * Girl Genius
 * Plays... oddly when Gilgamesh Wulfenbach is led to believe that Agatha was killed by a rogue clank. He begins the wind-up to a Big No before cold logic takes over. However, in a flashback to the same scene, Gil is in the classic "Big No" pose. So it's unclear whether he did one or not.
 * Many Girl Genius characters get a Big No, of course. Gil demonstrates correct form here.
 * Candi presents the rare quixotically hopeful drunken ex-boyfriend version.
 * Silent Hill Promise: When Vanessa is grabbed into a pit by a...thing.
 * Today Nothing Happened: This very wiki is responsible in this strip.
 * Bob the Angry Flower has it at the end of this strip.
 * Used pretty straight in Final Blasphemy here and later in the same issue.
 * In Starslip Crisis, Zillion can't even do this right.
 * In The Inexplicable Adventures of Bob when Bob learns his roof is about to be destroyed a third time. It will happen many more times.
 * Riboflavin gets one when he faces a fate worse than death.
 * From Multiplex, sarcastically, from Kurt.
 * Candice didn't appreciate his creative marriage proposal.
 * Happens in Corner Alley 13 when Noelle notices that her café doesn't have any customers.
 * Right here in Elven Lacryment.
 * Dragon Mango spoofs this when Pumpkin's father returns after a four-year journey into faraway lands and gives Mango a rare treat, called a "sweet swirl stick", which Cherry eats in one bite.
 * Jason from Something Positive has a fairly unique one when realizing he's just turned down a 3-way with his wife and hot friend.
 * Rhea of Slightly Damned's reaction upon learning that she cannot be reincarnated.
 * Powerpuff Girls Doujinshi has the following conversation.

Professor Utonium: NNNNNOOOOOOOOOOOOOOOOOOOOOOOO!!!"


 * A Nooooo! that bursts out of the panel in Jayden and Crusader.
 * Cale whips one out in this Looking for Group comic.
 * DMFA has this beauty when a young Abel learns that extra lessons include extra homework.
 * Penny Arcade: Gabriel provides this gem upon besting Tycho in a card game.
 * In Brawl in the Family, Kirby unleashes one when Meta Knight takes his revenge on him here.
 * Monica from Wapsi Square had a nice one when
 * The Princess of Rusty and Co when she found the White Knight crushed.
 * Loserz parodied the original scene here.
 * Dork Tower audible five miles away
 * Chris in Sonichu does this when he learns that a Starbucks barista only went out with him as a joke. Apparently, this happened in real life.
 * Nith from What's Shakin discovers his home has been destroyed.
 * ''Ménage à 3': Kiley here.
 * Zii's reaction when Di Di tries to cut her hair into a mullet.
 * Zii here.
 * Kiley again here when she thinks Yuki is going to see her father in Japan when she's in a bad condition.
 * Kiley yet again here.
 * Mulberry
 * Mulberry said one when one comic ended with her
 * She performed another one after Jack showed her a newspaper article detailing the death of Brittany Murphy.
 * Princess Pi
 * Alpha Bitch Sam yells this after getting sentenced to  as punishment for blowing up Piscataway Stadium.
 * Princess Pi herself says this when the new princess, Gertie, makes her genie, Bottle Blonde, encase her own bottle in concrete, so that Pi could not make Bottle Blonde turn Piscataway back to normal.
 * The Platypus Comix story "True Believers" had one after.
 * In Shifters Ben Harkus gives us one of these here after
 * Bulbasaur, after dropping a little girl in a failed rescue attempt in 151 Hidden Depths.
 * In Cucumber Quest, Cucumber delivers one as Sir Tomato brings his sword down on him.